Magnefect-nano II: Cell Transfection Device

Integrated Scientific Solutions Inc. » Lab Products » Cell Biology » Cell Transfection » Magnefect-nano II: Cell Transfection Device

Transfecting hard-to-transfect cells (e.g. neuronal and primary neuronal cells) and tissues is a problem for many researchers.  The ability to penetrate physical barriers to transfection, such as mucous-coated lung epithelial cells, offers potential advancements for therapeutic applications in genetic disorders such as cystic fibrosis.  The Magnefect-nano II system uses a patented technology which applies proprietary oscillating magnet arrays to plated cells, resulting in significantly improved transfection speed and efficiency.  Researchers are able to transfect their adherent cells without trypsinization, resulting in healthier, happier cells.  This results in significantly faster and higher transfection efficiencies over the traditionally used lipid-based and static magnet assisted transfection platforms, as well as dramatically improved cell viability over electroporation methods.

Transfecting cells with Nanomagnetic Transfection is simple and highly efficient.  It is an easy-to-handle, fast and efficient technology using biomolecules, such as nucleic acids, associated with magnetic nanoparticles.  

The Magnefect-nano II is comprised of two very compact components:

  • The sample holder comes with three fully interchangeable magnet arrays (6-well, 24-well AND 96-well) for standard multi-well plates. The sample holder may be placed in a cell incubator during transfection to maintain the normal cell culture environment.
  • The controller unit supplies power to oscillate the magnet array and allows you to define user protocols (e.g. oscillating frequency, oscillating time, oscillating displacement). The magnefect-nano-II controller unit can also be used to provide power to a magnefect-nano-II-DUO sample holder.

Advantages versus alternative transfection methods: (Electroporation, Static Magnet Platforms and lipid-based methods)

  • High transfection efficiencies of nucleic acids (including plasmid DNA, siRNA and oligonucleotides) with a wide range of cell lines and cell types, including hard-to-transfect cells such as primary cells.
  • Low cytotoxicity and no adverse effects on the viability of transfected cells at the recommended vector doses.
  • Higher levels of protein expression.
  • Transgene expression levels increased up to several thousand fold after short-term transfection in comparison to lipid-based reagents.
  • Easy, fast (
  • Functional with serum and serum-free.
  • Cost-effective; saving time and materials

Magnefect-nano II Product Brochure

Primary Neuron Brochure

Neuronal Data Brochure

Nanotherics Website

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Technical Details

How it works:

  • Biomolecules, such as nucleic acids or other molecules, are bound to magnetic nanoparticles made of biodegradable iron oxides coated with cationic molecules in a simple one-step process.
  • A magnetic force is applied beneath the cells to be transfected. This draws the magnetic nanoparticle and associated biomolecule onto cells on the bottom of the multi-well plate or cell culture flask used. The full nucleic acid dose is drawn toward and delivered into target cells generally via endocytosis. This leads to rapid and efficient transfection without disturbing membrane architecture and without causing chromosomal damage or leaving holes in cell membranes. This is in contrast to other physical transfection methods that damage, create holes or electroshock the cell membranes.
  • The magnetic nanoparticles are biodegradable and non-toxic at the recommended doses.
  • In this manner, the complete vector dose is concentrated on the cells within minutes and 100% of the cells are generally in contact with significant vector dose.
  • The nucleic acids or other vectors are then released into the cytoplasm by different mechanisms depending on the formulation used.
  • In the case of the nanoTherics technology the magnetic field that is created is oscillating in nature which promotes more efficient uptake, resulting in transfection efficiencies even higher than static-based magnet assisted transfection techniques.

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